Application of DNA Microarray Technology for Wastewater Analysis - C. Maynard, L. Masson, F. Berthiaume - diskuze

Deoxyribonucleic acid (DNA) microarrays are widely used for differential expression studies and for detection of virulence genes in pure bacterial cultures. Their use in complex microbial samples, such as soil and wastewater, has been relatively less studied. This report presents the results of a systematic effort to apply DNA microarrays to pathogen detection and to bacterial source tracking (BST) in wastewater. Parameters such as the method of DNA extraction from the samples, the type of immobilized probe (whether polymerase chain reaction [PCR] amplicons or oligonucleotides), the length and method of immobilization of oligonucleotides, the method of DNA labelling, the combination of PCR amplification with microarray hybridization and the choice of PCR targets have been optimized. Results indicate that the combination of PCR followed by microarray hybridization can detect pathogens in wastewater samples down to a 0.1% detection limit. The use of microarrays for bacterial source tracking gave promising results on human samples, however the probes used in this study only provided signals for general indicators of fecal contamination when used on samples of animal origin. The overall conclusion is that microarray technology has not yet reached the stage of routine use for microbiological analysis of wastewater. This report demonstrates: That the hybridization of total genomic DNA on microarrays has a high detection limit, of the order of 10^7 genomes; That the use o
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